Draft genome sequence of a carbapenemase-producing (NDM-1) and multidrug-resistant, hypervirulent Klebsiella pneumoniae ST11 isolate from Pakistan, with a non-hypermucoviscous phenotype associated with rmpA2 mutation

ST11 is a high-risk sequence type associated with carbapenem-resistant Klebsiella pneumoniae strains. Carbapenemase-producing hypervirulent K. (hvKp) are major concern as they harbour diverse range of pathogenicity traits. Here we describe the characteristics strain KP75w isolated from tertiary-care hospital in Pakistan. Antimicrobial susceptibility testing was performed by Kirby–Bauer disk diffusion test and broth microdilution assay. The virulence phenotype determined string well biofilm cell adhesion assays. Genome sequencing using MiSeq HiSeq 2500 platforms 30 × coverage. resistance profiling characterised multidrug-resistant carbapenemase-producing meropenem minimum inhibitory concentration (MIC) 4 μg/mL, which above CLSI susceptible breakpoint (≤1 μg/mL). annotated contigs indicated genome size 5 644 609 bp 5679 coding regions. (ST11) designated hvKp on basis presence carbapenemase gene (blaNDM-1) hypervirulence genes (rmpA2, iucABCD–iutA, fyuA, irp, mrk, ybt, fep virB2). found to contain 163-kb region showing 58.8% identity large plasmid pLVPK, supporting KP75w. novel non-hypermucoviscous that appears represent convergence multidrug traits clinical strains Southeast Asian region.